Nkhani - Chilengedwe Vanillin Celsiders

I.untroduckecction

Vanillin ndi amodzi mwa omwe amagwiritsidwa ntchito kwambiri ndipo amagwiritsidwa ntchito kwambiri padziko lapansi. Pachikhalidwe, amachokera ku Nyeta nyemba, zomwe ndizodula ndikukumana ndi zovuta zokhudzana ndi kukhazikika ndikupereka chinsinsi. Komabe, ndikupita patsogolo kwa Biotechnology, makamaka mu gawo la mabiobial biotransfor, nthawi yatsopano ya chilengedwe chayamba. Kugwiritsa ntchito tizilombo tatiologis kusinthidwe kwachilengedwe zomwe zimapangitsa kuti pakhale njira yothandizira pachuma cha synthesis of Vanillin. Njira imeneyi siyongowonjezera nkhawa komanso imaperekanso njira zothetsera mafakitale. Kufufuza komwe kudachitika ndi SRM Institute of Science ndi Ternology (Smist) kwaperekanso njira zosiyanasiyana zothandizira kuphatikiza kwazakudya kuchokera mumiyala yazakudya komanso kugwiritsa ntchito njira zosiyanasiyana pamakampani ogulitsa zakudya.

Ii. Momwe Mungapezere Vanillin Vallin kuchokera ku zinthu zokonzanso

Kugwiritsa ntchito acid acid ngati gawo lapansi

Ferlialic acid, ochokera ku magwero ngati mpunga wa mpunga ndi oat, akuwonetsa zojambulajambula zopangidwa ndi sillillin ndipo amagwira ntchito ngati yogwiritsa ntchito popanga mankhwala. Microorganism zosiyanasiyana monga pseudomonas, asrgullonas, zokopa, ndi bowa adalemba ntchito kuti apangike ku Verlianic acid. Zoyenera, mitundu ngati amycolatopsis ndi mafangashi ozungulira adziwika kuti ofuna kupanga vallin kuchokera ku Ferhinic acid. Kafukufuku angapo afufuza ku Vallillin kuchokera ku Ferhinic acid pogwiritsa ntchito tizilombo tating'onoting'ono, njira zosinthira, komanso njira zosasinthika, ndikuwonetsa kusintha kwa njirayi.

Syzymatic synthesis of vallillin kuchokera ku firulic acid imaphatikizapo kiyi enzyme Ferzyl, omwe amakopa hydrolysis a antersic acid, kumasula Vallillin ndi zina zokhudzana ndi zinthu zina. Pakufufuza kuchuluka kwakukulu kwa michere ya vallillin yopanda ma cell, ofufuza apanga zobwezeretsera zowongolera zosintha bwino Estherichia colin strain amatha kutembenuza acid a acid (20mm). Kuphatikiza apo, kugwiritsa ntchito ma cell a cell calobiliazation kumadziyang'anitsitsa chifukwa cha zinthu zake zabwino komanso zokhazikika pansi pa mikhalidwe zosiyanasiyana. Njira Yosasinthika Yachikulu Yopanga Vallillin kupanga brulic acid adapangidwa, kuthetsa kufunika kwa mgwirizano wogwirizana. Njira imeneyi imaphatikizapo ma coenzyme odziyimira payekha ndi ma coenzyme-pawokha oxygenase omwe amabwera chifukwa cha kutembenuka kwa Fersic acid ku Vallianin. The Iskondabilization wa FDC ndi CSO2 imathandizira kupanga kwa 2.5 mg ya vallillin kuchokera ku firulic acid m'magazini a centillinized enzyme biotechnology.

Kugwiritsa ntchito Eugeeni / Isoogenol ngati gawo lapansi

Eugeenol ndi isoougenol, atachitidwa ku Bayocnougenol, amapanga zolakwa ndi metabolites yake, yomwe yapezeka kuti ikugwira ntchito zosiyanasiyana komanso phindu lachuma. Kafukufuku angapo asinthasintha kugwiritsa ntchito kosinthika komanso mwachilengedwe kuchitika tizilombo tating'onoting'ono tosiyanasiyana ku Valuenin kuchokera ku eugenol. Kuthekera kwa kuwonongeka kwa eugenol kwawonedwa m'mabakiteriya osiyanasiyana ndi bowa, kuphatikiza koma osakhala ndi bacillus, maspergilyonas, a asrgullus, ndi rhodococcus. Kugwiritsa ntchito kwa eugenol oxidase (eugo) ngati enzyme yopanga mafakitale m'malo ogulitsa mafakitale yawonetsa kuthekera kotheka. Eugo amawonetsa kukhazikika komanso ntchito pazinthu zochulukirapo, ndi kusungunuka zochulukitsa ntchito ndikuchepetsa nthawi. Kuphatikiza apo, kugwiritsa ntchito eugo kokhazikika kumapangitsa kuti kuchira kwa biocotalyst mpaka kuzungulira kuzungulira kwa mizere 18 yopitilira, kumapangitsa kuwonjezeka kopitilira 12 biocatalyst. Mofananamo, enzyme yopanda chipani cha CSO2 imatha kulimbikitsa kutembenuka kwa isoougenol ku Vanillin popanda kudalirana ndi ma coenzymes.

Magawo ena

Kuphatikiza pa Ferlialic acid ndi eugeenol, zinthu zina monga vanillic acid ndi C3-C3 Phnylpropanoids zomwe zadziwika kuti ndizotheka kupanga. Vanillic acid, yopangidwa ngati chinthu cha Lignin kuwonongeka kapena ngati gawo lopikisana mu ma metabolic njira, limawerengedwa kuti ndi malo ofunikira a bio-roclectin. Kuphatikiza apo, kupereka chidziwitso pakugwiritsa ntchito C6-C3 Phnylpropanoids kwa vallillin synthesis kumapereka mwayi wapadera wokhala ndi zipatso zatsopano komanso zatsopano.

Pomaliza. Njira imeneyi imapereka njira ina yopangira Vanillin, kuthana ndi mavuto osokoneza bongo komanso kuchepetsa kudalira chikhalidwe chodunitsira miyambo. Mapulogalamu osiyanasiyana ndi mtengo wachuma wa Vallilnin kudutsa malonda ogulitsa zakudya amatsimikizira kufunika kopitilira kufufuza komanso chitukuko m'derali. Kukula kwa mtsogolo ku gawo la chilengedwe ku Vanillin Vadillin kupanga kumatha kusintha mafakitale a kununkhira, kupereka njira zina zokhazikika komanso zopatsa thanzi za kununkhira kwatsopano. Tikamayesetsa kutsatira zomwe zingatheke kuchita zinthu zofunika kwambiri komanso zochitika zapamwamba, kupanga kwa zida za chilengedwe kuchokera m'malo osiyanasiyana kumapereka njira yabwino kwambiri yochitira nsanje.

Iii. Kodi maubwino ogwiritsa ntchito zinthu zokonzanso ntchito kuti apange ku Vallin Villin

Zachilengedwe:Kugwiritsa ntchito zida zokonzanso monga zomera ndi zinyalala zopangira zotchinga zitha kuchepetsa kufunikira kwa mafuta osungirako zinthu zakale, kuchepetsa zovuta zachilengedwe, ndikuchepetsa mpweya woipa.

Kukhazikika:Kugwiritsa ntchito zinthu zokonzanso kumathandizira mphamvu yokhazikika komanso zopangira, kuthandiza kuteteza zinthu zachilengedwe ndikukwaniritsa zosowa zam'mibadwo yamtsogolo.

Chitetezo cha Zamoyo:Kugwiritsa ntchito bwino zinthu zambiri, zomera zamtchire zimatha kutetezedwa, zomwe zimathandizira chitetezo cha zachilengedwe komanso kukonza chilengedwe.

Zogulitsa:Poyerekeza ndi zopangidwa ndi sivillin, vidial villillin amatha kukhala ndi zabwino zambiri mu mtundu wa fungo labwino komanso mawonekedwe achilengedwe, omwe angakuthandizeni kukonza mtundu wa kununkhira komanso kununkhira kwa zonunkhira.

Chepetsani kudalira pamafuta oyambira:Kugwiritsa ntchito zinthu zokonzanso kumathandizanso kudalirika chifukwa cha mafuta ochepa, omwe ndi opindulitsa chitetezo champhamvu ndi mphamvu zosiyanasiyana. Tikukhulupirira kuti chidziwitso pamwambapa chitha kuyankha mafunso anu. Ngati mukufuna chikalata chowerengera mu Chingerezi, chonde dziwitsani kuti nditha kukupatsirani.

Iv. Mapeto

Kutha kugwiritsa ntchito zinthu zokonzanso kuti apange villil villin ngati njira yokhazikika komanso yosangalatsa zachilengedwe ndikofunikira. Njira imeneyi imalonjeza kuti ikuthana ndi kufunika kwa chilengedwe ku Valliallin ndikuchepetsa kudalira njira zopangira zopangira zopanga.

Vidiallin yachilengedwe imakhala ndi malo ofunikira mu malonda, olemekezeka chifukwa cha kununkhira kwake komanso kugwiritsa ntchito kofala ngati wothandizika m'malo osiyanasiyana. Ndikofunikira kutsindika za kufunikira kwa chilengedwe monga chofufutira pambuyo pa chakudya, chakumwa, komanso mafakitale onunkhira chifukwa cha zokonda zake zapamwamba komanso zokonda zachilengedwe.

Kuphatikiza apo, gawo la chilengedwe ku Valliallin limapereka mwayi waukulu wofufuza komanso kukula. Izi zikuphatikiza kufufuzanira matekinoloji atsopano komanso njira zatsopano kuti muwonjezere bwino ntchito ndi kukhazikika kwa kupanga zachilengedwe kuchokera ku zida zapamwamba. Kuphatikiza apo, kusintha kwa njira zopangira zopangira ndalama ndi kuchuluka kwa ndalama kumathandizira pakulimbikitsa kutengera kwachilengedwe ku Valliallin zachilengedwe ngati njira yokhazikika komanso yosangalatsa.